The SCSB X-ray Crystallography Laboratory is located on the 6th floor of
the Libby Moody Thompson Basic Science Building. Its mission is to provide
access to state of the art X-ray diffraction instrumentation and the
associated support facilities for crystallographic research at UTMB.
The Center has recently installed new x-ray diffraction and
solution scattering instrumentation for the lab. The new equipment starts
with the Rigaku Ultimate Home Lab which comprises the unsurpassed high-brilliance
FR-E++DW Superbright x-ray generator, with the industry standard RAXIS-IV++
crystallography system with both Cu and Cr optics. In addition, we have acquired the
innovative Rigaku BioSAXS-1000 with a 96-well automatic sample changer. This is the first
SAXS instrument dedicated to biological research in the southern mid-western states, once
again marking UTMB as a leader in structural biology resources.
The SCSB X-ray Crystallography resources
currently consists of two x-ray area detector systems. The first area detector is a
Rigaku R-Axis-IV++ dual
30cm Imaging Plate detector mounted on the Rigaku FR-E++DW Superbright x-ray generator.
The second detector is a Brüker
D8 Venture with
the high intensity TURBO TXS source, which can reach upto 0.84 Å resolution. For
enhanced data we have available a choice of sample cooling systems. Both systems are
equipped with Crysosystems, the R-AXIS a Cryo Industries of America CRYOCOOLER, the Brüker
an Oxford Cryostream 800. A 4 °C Cold Air refrigerated cooling system is available for
samples which cannot be frozen, or do not require freezing.
The Rigaku BioSAXS-1000 2D-Kratky camera is mounted on the left port of our
Rigaku FR-E++DW Superbright x-ray generator. The camera is equiped with a
96-well automatic sample changer (ASC-96) with an in-vacu quartz flow-cell. In addition,
we have several quartz sample cells for manual loading of smaller sample volumes or
detergent samples, which are not compatible with the ASC-96 liquid handling system. The
quality of SAXS data obtained with the BioSAXS camera has been exceptional. Using our
data processing and
analysis the optimal quality data can be collected. It is not unusual to collect
useful data to the limit of the detector (q~0.7 Å-1). This camera offers unsurpassed
solution scattering data for macromolecular samples. For more information visit our SAXNS site.