The SCSB Cryo-electron Microscopy (Cryo-EM) Laboratory, located on the first floor of the Medical Research Building, features a BSL-3 containment for viral and pathogen work.
In addition, the laboratory has an open research space where structure of macromolecules, their complexes, cell organelles, and other biological systems can be studied using various EM techniques, including cryo-electron microscopy and (cryo-) electron tomography. The laboratory has two modern JEOL cryo-electron microscopes: a state-of-the-art 200 keV JEOL 2200FS cryo-EM with in-column electron energy filter and field emission gun (the brightest electron source available today), and a 200 keV JEOL 2100 EM.
The high-resolution 200 keV JEM2200FS is located in the W. M. Keck Center for virus imaging in BSL-3 containment and permits the safe structural imaging of highly infectious pathogens that could not studied in the open research area. This is the first cryo-EM facility in the US designed for high-resolution structural studies of wild type infectious agents. The microscope can be controlled remotely through a computer network, which provides access for remote online users and largely extends our user base.
The JEM2100 is available for imaging of non-pathogenic specimens and negatively stained samples. All microscopes in the Laboratory are equipped for (cryo-) electron tomography using automated data collection procedure. The JEM2100 is used for both user training and structural studies. A shared Crystallographic/EM Computational Lab provides high-throughput image-processing with a dedicated 120-core EM Image-Processing Cluster. Images are stored in a local EMEN database that is archived remotely.