Cryo-electron Microscopy (Cryo-EM)

Including the W. M. Keck Center for Virus Imaging in BSL-3

The SCSB Cryo-electron Microscopy (Cryo-EM) Laboratory, located on the first floor of the Medical Research Building, features a BSL-3 containment for viral and pathogen work. In addition, the laboratory has an open research space where structure of macromolecules, their complexes, cell organelles, and other biological systems can be studied using various EM techniques, including cryo-electron microscopy and (cryo-) electron tomography. The laboratory has three modern JEOL cryo-electron microscopes : a state-of-the-art 200 keV JEOL 2200FS cryo-EM with in-column electron energy filter and field emission gun (the brightest electron source available today), a 200 keV JEOL 2100 EM, and a 120 keV JEM1400 microscope.

The high-resolution 200 keV JEM2200FS is located in the W. M. Keck Center for virus imaging in BSL-3 containment and permits the safe structural imaging of highly infectious pathogens that could not studied in the open research area. This is the first cryo-EM facility in the US designed for high-resolution structural studies of wild type infectious agents. The microscope can be controlled remotely through a computer network, which provides access for remote online users and largely extends our user base.

The JEM2100 is available for imaging of non-pathogenic specimens. The 120 keV JEM1400 microscope is available for imaging of negatively stained samples, thin and semi-thick plastic cell- and tissue sections and cryo-electron microscopy. All microscopes in the Laboratory are equipped for (cryo-) electron tomography using automated data collection procedure. The JEM1400 is used for both user training and structural studies. A shared Crystallographic/EM Computational Lab provides high-throughput image-processing with a dedicated 120-core EM Image-Processing Cluster. Images are stored in a local EMEN database that is archived remotely.

For more information please contact the SCSBMB Cryo-EM manager.

Manager: Misha Sherman, PhD
Tel: (409) 772-6310

Research Scientist: Michael Woodson, PhD
Tel: (409) 772-6327

Cyro-EM Intranet


Prof. Misha Sherman Prof. Misha Sherman Prof. Marc Morais Prof. Marc Morais Prof. Kay Choi Prof. Kay Choi
Dr. Krill Grushin Dr. Krill Grushin Dr. Mitul Saha (with Prof. Morais) Dr. Mitul Saha (with Prof. Morais) Dr. Michael Woodson Dr. Michael Woodson
Emilio Reyes-Aldrete Emilio Reyes-Aldrete